Publications

“Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction”

Ellis, J.M.; Campbell, M.E.; Kumar, P.; Geunes, E.P.; Bingman, C.A.; Buller, A.R. ChemRxiv. 2021

“De novo biosynthesis of a nonnatural cobalt porphyrin cofactor in E. coli and incorporation into hemoproteins”

Perkins, L.J.; Weaver, B.R.; Buller, A.R.; Burstyn, J.N. PNAS. 2021

https://www.pnas.org/content/118/16/e2017625118

“L-Threonine transaldolase activity is enabled by a persistent catalytic intermediate”

Kumar, P.; Meza, A.; Ellis, J. M.; Carlson, G. A.; Bingman, C. A.; Buller, A. R. ACS Chemical Biology. 2020

https://doi.org/10.1021/acschembio.0c00753

“Modular control of L-tryptophan isotopic substitution via an efficient biosynthetic cascade”

Thompson, C.; McDonald, A.; Yang, H.; Cavagnero, S.; Buller, A. R. Organic & Biomolecular Chemistry. 2020

https://doi.org/10.1039/D0OB00868K

“Facile in vitro biocatalytic production of diverse tryptamines”

McDonald, A.; Perkins, L.; Buller, A. R. ChemBioChem 2019.

https://doi.org/10.1002/cbic.201900069

Prof. Buller’s previous publications

2020

  • Beasley, M., Lumley, M., Janicki, T., Fernandez, R., Manger, L., Tucholski, T., Thomas, N., Whitmire, L., Lawson, A., Buller, A.R. J. Chem. Educ. 2020, 97, 3, 643–650. Student-Led Climate Assessment Promotes a Healthier Graduate School Environment. https://doi.org/10.1021/acs.jchemed.9b00611

2019

  • Patel KD, d’Andrea FB, Gaudelli NM, Buller AR, Townsend CA, Gulick AM. Nat Commun. 2019 Aug 27;10(1):3868. Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions. doi.org/10.1038/s41467-019-11740-6

2018

  • Kan, S. B. J.; Garcia-Borràs, M.; Lewis, R. D.; Houk, K. N.; Chalkley, M. J.; Buller, A. R.; Arnold, F. H. Proc. Natl. Acad. Sci. 2018, 115 (28), 7308. doi.org/10.1073/pnas.1807027115
  • Buller A.R, van Roye P., Cahn J.KB, Scheele R.A, Herger M., Arnold F.H.  2018.  Directed Evolution Mimics Allosteric Activation by Stepwise Tuning of the Conformational Ensemble. Journal of the American Chemical Society. doi.org/10.1021/jacs.8b03490
  • Boville C.E, Scheele R.A, Koch P., Brinkmann-Chen S., Buller A.R, Arnold F.H.  2018.  Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues. Angewandte Chemie-International Edition. 57:14764-14768.  doi.org/10.1002/anie.201807998

2017

  • Prier C.K, Zhang R.K, Buller A.R, Brinkmann-Chen S., Arnold F.H.  2017.  Enantioselective, intermolecular benzylic C-H amination catalysed by an engineered iron-haem enzyme. Nature Chemistry. 9:629-634. doi.org/10.1038/nchem.2783

2016

  • Cahn J.KB, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  Artificial domain duplication replicates evolutionary history of ketol-acid reductoisomerases. Protein ScienceProtein Science. 25:1241-1248. doi.org/10.1002/pro.2852
  • Murciano-Calles J., Romney D.K, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  A Panel of TrpB Biocatalysts Derived from Tryptophan Synthase through the Transfer of Mutations that Mimic Allosteric Activation. Angewandte Chemie-International Edition. 55:11577-11581. doi.org/10.1002/anie.201606242
  • Herger M., van Roye P., Romney D.K, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  Synthesis of beta-Branched Tryptophan Analogues Using an Engineered Subunit of Tryptophan Synthase. Journal of the American Chemical Society. 138:8388-8391. doi.org/10.1021/jacs.6b04836 
  • Buller A.R, van Roye P., Murciano-Calles J., Arnold F.H.  2016.  Tryptophan Synthase Uses an Atypical Mechanism To Achieve Substrate Specificity. Biochemistry. 55:7043-7046. doi.org/10.1021/acs.biochem.6b01127

2015

  • Cahn J.KB, Brinkmann-Chen S., Spatzal T., Wiig J.A, Buller A.R, Einsle O., Hu Y.L, Ribbe M.W, Arnold F.H.  2015.  Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases. Biochemical Journal. 468:475-484. doi.org/10.1042/bj20150183
  • Marous D.R, Lloyd E.P, Buller A.R, Moshos K.A, Grove T.L, Blaszczyk A.J, Booker S.J, Townsend C.A.  2015.  Consecutive radical S-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 112:10354-10358. doi.org/10.1073/pnas.1508615112
  • Buller A.R, Brinkmann-Chen S., Romney D.K, Herger M., Murciano-Calles J., Arnold F.H.  2015.  Directed evolution of the tryptophan synthase beta-subunit for stand-alone function recapitulates allosteric activation. Proceedings of the National Academy of Sciences of the United States of America. 112:14599-14604. doi.org/10.1073/pnas.1516401112
  • McIntosh J.A, Heel T., Buller A.R, Chio L., Arnold F.H.  2015.  Structural Adaptability Facilitates Histidine Heme Ligation in a Cytochrome P450. Journal of the American Chemical Society. 137:13861-13865. doi.org/10.1021/jacs.5b07107

2014

  • Hyster T.K, Farwell C.C, Buller A.R, McIntosh J.A, Arnold F.H.  2014.  Enzyme-Controlled Nitrogen-Atom Transfer Enables Regiodivergent C-H Amination. Journal of the American Chemical Society. 136:15505-15508. doi.org/10.1021/ja509308v
  • Buller A.R, Freeman M.F, Schildbach J.F, Townsend C.A.  2014.  Exploring the Role of Conformational Heterogeneity in cis-Autoproteolytic Activation of ThnT. Biochemistry. 53:4273-4281. doi.org/10.1021/bi500385d

2013

  • Buller A.R, Townsend C.A.  2013.  Intrinsic evolutionary constraints on protease structure, enzyme acylation, and the identity of the catalytic triad. Proceedings of the National Academy of Sciences of the United States of America. 110:E653-E661. doi.org/10.1073/pnas.1221050110

2012

  • Buller A.R, Labonte J.W, Freeman M.F, Wright N.T, Schildbach J.F, Townsend C.A.  2012.  Autoproteolytic Activation of ThnT Results in Structural Reorganization Necessary for Substrate Binding and Catalysis. Journal of Molecular Biology. 422:508-518. doi.org/10.1016/j.jmb.2012.06.012
  • Buller A.R, Freeman M.F, Wright N.T, Schildbach J.F, Townsend C.A.  2012.  Insights into cis-autoproteolysis reveal a reactive state formed through conformational rearrangement. Proceedings of the National Academy of Sciences of the United States of America. 109:2308-2313. doi.org/10.1073/pnas.1113633109

2008

  • Bassuk A.G, Wallace R.H, Buhr A., Buller A.R, Afawi Z., Shimojo M., Miyata S., Chen S., Gonzalez-Alegre P., Griesbach H.L et al..  2008.  A Homozygous Mutation in Human PRICKLE1 Causes an Autosomal-Recessive Progressive Myoclonus Epilepsy-Ataxia Syndrome. American Journal of Human Genetics. 83:572-581. doi.org/10.1016/j.ajhg.2008.10.003