Publications

“Substrate-Multiplexed Assessment of Aromatic Prenyltransferase Activity”

Peyton M. HigginsNicolette G. WehrliAndrew R. BullerChemBioChem 2024, e202400680.

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“Biocatalytic asymmetric aldol addition into unactivated ketones”

Bruffy, S.K., Meza, A., Soler, J. et al. Biocatalytic asymmetric aldol addition into unactivated ketones. Nat. Chem. (2024). https://doi.org/10.1038/s41557-024-01647-1

https://www.nature.com/articles/s41557-024-01647-1

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“Elucidation of the Stereochemical Mechanism of Cystathionine γ-Lyase Reveals How Substrate Specificity Constrains Catalysis”

Anna Zmich, Lydia J. Perkins, Craig Bingman, and Andrew R. Buller. ACS Catalysis 2024 14 (15), 11196-11204
DOI: 10.1021/acscatal.4c02281

https://pubs.acs.org/doi/10.1021/acscatal.4c02281

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“Molecular Determinants of Efficient Cobalt-Substituted Hemoprotein in E. coli

Weaver, B.R.; Perkins, L.J.; Fernandez Candelaria, F.O.; Burstyn, J.N.; Buller, A.R. ACS Synth. Biol. 2023.

https://pubs.acs.org/doi/10.1021/acssynbio.3c00481

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“Engineered Biocatalytic Synthesis of β-N-Substituted-⍺-Amino Acids”

Villalona, J.; Higgins, P.M.; Buller, A.R. Angewandte Chemie. 2023, e202311

https://onlinelibrary.wiley.com/doi/full/10.1002/anie.202311189

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“Multiplexed Assessment of Promiscuous Non-Canonical Amino Acid Synthase Activity in a Pyridoxal Phosphate-Dependent Protein Family”

Zmich, A.; Perkins, L.J.; Bingman, C.; Acheson, J.F.; Buller, A.R. ACS Catal. 2023, 11644–11655

https://pubs.acs.org/doi/10.1021/acscatal.3c02498

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“Engineering Enzyme Substrate Scope Complementarity for Promiscuous Cascade Synthesis of 1,2-Amino Alcohols”

McDonald, A.D.; Bruffy, S.K.; Kasat, A.T.; Buller, A.R. Angewandte Chemie. 2022, e202212637

https://onlinelibrary.wiley.com/doi/full/10.1002/anie.202212637

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“Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis”

McDonald, A.D.; Higgins, P.M.; Buller, A.R. Nat Comm. 2022, 13, 5242

https://www.nature.com/articles/s41467-022-32789-w

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Previously posted on ChemRxiv https://chemrxiv.org/engage/chemrxiv/article-details/618da1579960f3d941a8bed9

“Efficient Chemoenzymatic Synthesis of α-Aryl Aldehydes as Intermediates in C–C Bond Forming Biocatalytic Cascades”

Meza, A.; Campbell, M.E.; Zmich, A.; Thein, S.A; Grieger, A.M.; McGill, M.J.; Willoughby, P.H.; Buller, A.R. ACS Catal. 2022, 12, XXX, 10700–10710

https://pubs.acs.org/doi/10.1021/acscatal.2c02369?ref=PDF

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“Investigation of β-substitution activity of O-acetylserine sulfhydrolase from Citrullus vulgaris”

Smith, J.L.;  Harrison, I.M.; Bingman, C.; Buller, A.R. ChemBioChem. 2022. Accepted

https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202200157

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Site-selective deuteration of amino acids through dual-protein catalysis”

Doyon, T.J.; Buller, A.R. J. Am. Chem. Soc. 2022. 144, 16, 7327-7336.

https://pubs.acs.org/doi/10.1021/jacs.2c00608

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“Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction”

Ellis, J.M.; Campbell, M.E.; Kumar, P.; Geunes, E.P.; Bingman, C.A.; Buller, A.R. Nat Catal. 5, 136–143 (2022)

“Scalable and selective β-hydroxy-α-amino acid synthesis catalyzed by promiscuous L-threonine transaldolase ObiH”

Doyon, T.J.; Kumar, P.; Thein, S.; Kim, M.; Stitgen, A.; Grieger, A.M.; Madigan, C.; Willoughby, P.H.; Buller, A.R. ChemBioChem. 2021

https://doi.org/10.1002/cbic.202100577

“De novo biosynthesis of a nonnatural cobalt porphyrin cofactor in E. coli and incorporation into hemoproteins”

Perkins, L.J.; Weaver, B.R.; Buller, A.R.; Burstyn, J.N. PNAS. 2021

https://www.pnas.org/content/118/16/e2017625118

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“L-Threonine transaldolase activity is enabled by a persistent catalytic intermediate”

Kumar, P.; Meza, A.; Ellis, J. M.; Carlson, G. A.; Bingman, C. A.; Buller, A. R. ACS Chemical Biology. 2020

https://doi.org/10.1021/acschembio.0c00753

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“Modular control of L-tryptophan isotopic substitution via an efficient biosynthetic cascade”

Thompson, C.; McDonald, A.; Yang, H.; Cavagnero, S.; Buller, A. R. Organic & Biomolecular Chemistry. 2020

https://doi.org/10.1039/D0OB00868K

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“Facile in vitro biocatalytic production of diverse tryptamines”

McDonald, A.; Perkins, L.; Buller, A. R. ChemBioChem 2019.

https://doi.org/10.1002/cbic.201900069

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Other publications:

2020

  • Beasley, M., Lumley, M., Janicki, T., Fernandez, R., Manger, L., Tucholski, T., Thomas, N., Whitmire, L., Lawson, A., Buller, A.R. J. Chem. Educ. 2020, 97, 3, 643–650. Student-Led Climate Assessment Promotes a Healthier Graduate School Environment. https://doi.org/10.1021/acs.jchemed.9b00611

2019

  • Patel KD, d’Andrea FB, Gaudelli NM, Buller AR, Townsend CA, Gulick AM. Nat Commun. 2019 Aug 27;10(1):3868. Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions. doi.org/10.1038/s41467-019-11740-6

2018

  • Kan, S. B. J.; Garcia-Borràs, M.; Lewis, R. D.; Houk, K. N.; Chalkley, M. J.; Buller, A. R.; Arnold, F. H. Proc. Natl. Acad. Sci. 2018, 115 (28), 7308. doi.org/10.1073/pnas.1807027115
  • Buller A.R, van Roye P., Cahn J.KB, Scheele R.A, Herger M., Arnold F.H.  2018.  Directed Evolution Mimics Allosteric Activation by Stepwise Tuning of the Conformational Ensemble. Journal of the American Chemical Society. doi.org/10.1021/jacs.8b03490
  • Boville C.E, Scheele R.A, Koch P., Brinkmann-Chen S., Buller A.R, Arnold F.H.  2018.  Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues. Angewandte Chemie-International Edition. 57:14764-14768.  doi.org/10.1002/anie.201807998

2017

  • Prier C.K, Zhang R.K, Buller A.R, Brinkmann-Chen S., Arnold F.H.  2017.  Enantioselective, intermolecular benzylic C-H amination catalysed by an engineered iron-haem enzyme. Nature Chemistry. 9:629-634. doi.org/10.1038/nchem.2783

2016

  • Cahn J.KB, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  Artificial domain duplication replicates evolutionary history of ketol-acid reductoisomerases. Protein ScienceProtein Science. 25:1241-1248. doi.org/10.1002/pro.2852
  • Murciano-Calles J., Romney D.K, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  A Panel of TrpB Biocatalysts Derived from Tryptophan Synthase through the Transfer of Mutations that Mimic Allosteric Activation. Angewandte Chemie-International Edition. 55:11577-11581. doi.org/10.1002/anie.201606242
  • Herger M., van Roye P., Romney D.K, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  Synthesis of beta-Branched Tryptophan Analogues Using an Engineered Subunit of Tryptophan Synthase. Journal of the American Chemical Society. 138:8388-8391. doi.org/10.1021/jacs.6b04836 
  • Buller A.R, van Roye P., Murciano-Calles J., Arnold F.H.  2016.  Tryptophan Synthase Uses an Atypical Mechanism To Achieve Substrate Specificity. Biochemistry. 55:7043-7046. doi.org/10.1021/acs.biochem.6b01127

2015

  • Cahn J.KB, Brinkmann-Chen S., Spatzal T., Wiig J.A, Buller A.R, Einsle O., Hu Y.L, Ribbe M.W, Arnold F.H.  2015.  Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases. Biochemical Journal. 468:475-484. doi.org/10.1042/bj20150183
  • Marous D.R, Lloyd E.P, Buller A.R, Moshos K.A, Grove T.L, Blaszczyk A.J, Booker S.J, Townsend C.A.  2015.  Consecutive radical S-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 112:10354-10358. doi.org/10.1073/pnas.1508615112
  • Buller A.R, Brinkmann-Chen S., Romney D.K, Herger M., Murciano-Calles J., Arnold F.H.  2015.  Directed evolution of the tryptophan synthase beta-subunit for stand-alone function recapitulates allosteric activation. Proceedings of the National Academy of Sciences of the United States of America. 112:14599-14604. doi.org/10.1073/pnas.1516401112
  • McIntosh J.A, Heel T., Buller A.R, Chio L., Arnold F.H.  2015.  Structural Adaptability Facilitates Histidine Heme Ligation in a Cytochrome P450. Journal of the American Chemical Society. 137:13861-13865. doi.org/10.1021/jacs.5b07107

2014

  • Hyster T.K, Farwell C.C, Buller A.R, McIntosh J.A, Arnold F.H.  2014.  Enzyme-Controlled Nitrogen-Atom Transfer Enables Regiodivergent C-H Amination. Journal of the American Chemical Society. 136:15505-15508. doi.org/10.1021/ja509308v
  • Buller A.R, Freeman M.F, Schildbach J.F, Townsend C.A.  2014.  Exploring the Role of Conformational Heterogeneity in cis-Autoproteolytic Activation of ThnT. Biochemistry. 53:4273-4281. doi.org/10.1021/bi500385d

2013

  • Buller A.R, Townsend C.A.  2013.  Intrinsic evolutionary constraints on protease structure, enzyme acylation, and the identity of the catalytic triad. Proceedings of the National Academy of Sciences of the United States of America. 110:E653-E661. doi.org/10.1073/pnas.1221050110

2012

  • Buller A.R, Labonte J.W, Freeman M.F, Wright N.T, Schildbach J.F, Townsend C.A.  2012.  Autoproteolytic Activation of ThnT Results in Structural Reorganization Necessary for Substrate Binding and Catalysis. Journal of Molecular Biology. 422:508-518. doi.org/10.1016/j.jmb.2012.06.012
  • Buller A.R, Freeman M.F, Wright N.T, Schildbach J.F, Townsend C.A.  2012.  Insights into cis-autoproteolysis reveal a reactive state formed through conformational rearrangement. Proceedings of the National Academy of Sciences of the United States of America. 109:2308-2313. doi.org/10.1073/pnas.1113633109

2008

  • Bassuk A.G, Wallace R.H, Buhr A., Buller A.R, Afawi Z., Shimojo M., Miyata S., Chen S., Gonzalez-Alegre P., Griesbach H.L et al..  2008.  A Homozygous Mutation in Human PRICKLE1 Causes an Autosomal-Recessive Progressive Myoclonus Epilepsy-Ataxia Syndrome. American Journal of Human Genetics. 83:572-581. doi.org/10.1016/j.ajhg.2008.10.003