“Molecular Determinants of Efficient Cobalt-Substituted Hemoprotein in E. coli

Weaver, B.R.; Perkins, L.J.; Fernandez Candelaria, F.O.; Burstyn, J.N.; Buller, A.R. ACS Synth. Biol. 2023.


“Engineered Biocatalytic Synthesis of β-N-Substituted-⍺-Amino Acids”

Villalona, J.; Higgins, P.M.; Buller, A.R. Angewandte Chemie. 2023, e202311



“Multiplexed Assessment of Promiscuous Non-Canonical Amino Acid Synthase Activity in a Pyridoxal Phosphate-Dependent Protein Family”

Zmich, A.; Perkins, L.J.; Bingman, C.; Acheson, J.F.; Buller, A.R. ACS Catal. 2023, 11644–11655



“Engineering Enzyme Substrate Scope Complementarity for Promiscuous Cascade Synthesis of 1,2-Amino Alcohols”

McDonald, A.D.; Bruffy, S.K.; Kasat, A.T.; Buller, A.R. Angewandte Chemie. 2022, e202212637


“Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis”

McDonald, A.D.; Higgins, P.M.; Buller, A.R. Nat Comm. 2022, 13, 5242


Previously posted on ChemRxiv

“Efficient Chemoenzymatic Synthesis of α-Aryl Aldehydes as Intermediates in C–C Bond Forming Biocatalytic Cascades”

Meza, A.; Campbell, M.E.; Zmich, A.; Thein, S.A; Grieger, A.M.; McGill, M.J.; Willoughby, P.H.; Buller, A.R. ACS Catal. 2022, 12, XXX, 10700–10710


“Investigation of β-substitution activity of O-acetylserine sulfhydrolase from Citrullus vulgaris”

Smith, J.L.;  Harrison, I.M.; Bingman, C.; Buller, A.R. ChemBioChem. 2022. Accepted



Site-selective deuteration of amino acids through dual-protein catalysis”

Doyon, T.J.; Buller, A.R. J. Am. Chem. Soc. 2022. 144, 16, 7327-7336.


“Biocatalytic synthesis of non-standard amino acids by a decarboxylative aldol reaction”

Ellis, J.M.; Campbell, M.E.; Kumar, P.; Geunes, E.P.; Bingman, C.A.; Buller, A.R. Nat Catal. 5, 136–143 (2022)

“Scalable and selective β-hydroxy-α-amino acid synthesis catalyzed by promiscuous L-threonine transaldolase ObiH”

Doyon, T.J.; Kumar, P.; Thein, S.; Kim, M.; Stitgen, A.; Grieger, A.M.; Madigan, C.; Willoughby, P.H.; Buller, A.R. ChemBioChem. 2021

“De novo biosynthesis of a nonnatural cobalt porphyrin cofactor in E. coli and incorporation into hemoproteins”

Perkins, L.J.; Weaver, B.R.; Buller, A.R.; Burstyn, J.N. PNAS. 2021


“L-Threonine transaldolase activity is enabled by a persistent catalytic intermediate”

Kumar, P.; Meza, A.; Ellis, J. M.; Carlson, G. A.; Bingman, C. A.; Buller, A. R. ACS Chemical Biology. 2020


“Modular control of L-tryptophan isotopic substitution via an efficient biosynthetic cascade”

Thompson, C.; McDonald, A.; Yang, H.; Cavagnero, S.; Buller, A. R. Organic & Biomolecular Chemistry. 2020


“Facile in vitro biocatalytic production of diverse tryptamines”

McDonald, A.; Perkins, L.; Buller, A. R. ChemBioChem 2019.


Other publications:


  • Beasley, M., Lumley, M., Janicki, T., Fernandez, R., Manger, L., Tucholski, T., Thomas, N., Whitmire, L., Lawson, A., Buller, A.R. J. Chem. Educ. 2020, 97, 3, 643–650. Student-Led Climate Assessment Promotes a Healthier Graduate School Environment.


  • Patel KD, d’Andrea FB, Gaudelli NM, Buller AR, Townsend CA, Gulick AM. Nat Commun. 2019 Aug 27;10(1):3868. Structure of a bound peptide phosphonate reveals the mechanism of nocardicin bifunctional thioesterase epimerase-hydrolase half-reactions.


  • Kan, S. B. J.; Garcia-Borràs, M.; Lewis, R. D.; Houk, K. N.; Chalkley, M. J.; Buller, A. R.; Arnold, F. H. Proc. Natl. Acad. Sci. 2018, 115 (28), 7308.
  • Buller A.R, van Roye P., Cahn J.KB, Scheele R.A, Herger M., Arnold F.H.  2018.  Directed Evolution Mimics Allosteric Activation by Stepwise Tuning of the Conformational Ensemble. Journal of the American Chemical Society.
  • Boville C.E, Scheele R.A, Koch P., Brinkmann-Chen S., Buller A.R, Arnold F.H.  2018.  Engineered Biosynthesis of beta-Alkyl Tryptophan Analogues. Angewandte Chemie-International Edition. 57:14764-14768.


  • Prier C.K, Zhang R.K, Buller A.R, Brinkmann-Chen S., Arnold F.H.  2017.  Enantioselective, intermolecular benzylic C-H amination catalysed by an engineered iron-haem enzyme. Nature Chemistry. 9:629-634.


  • Cahn J.KB, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  Artificial domain duplication replicates evolutionary history of ketol-acid reductoisomerases. Protein ScienceProtein Science. 25:1241-1248.
  • Murciano-Calles J., Romney D.K, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  A Panel of TrpB Biocatalysts Derived from Tryptophan Synthase through the Transfer of Mutations that Mimic Allosteric Activation. Angewandte Chemie-International Edition. 55:11577-11581.
  • Herger M., van Roye P., Romney D.K, Brinkmann-Chen S., Buller A.R, Arnold F.H.  2016.  Synthesis of beta-Branched Tryptophan Analogues Using an Engineered Subunit of Tryptophan Synthase. Journal of the American Chemical Society. 138:8388-8391. 
  • Buller A.R, van Roye P., Murciano-Calles J., Arnold F.H.  2016.  Tryptophan Synthase Uses an Atypical Mechanism To Achieve Substrate Specificity. Biochemistry. 55:7043-7046.


  • Cahn J.KB, Brinkmann-Chen S., Spatzal T., Wiig J.A, Buller A.R, Einsle O., Hu Y.L, Ribbe M.W, Arnold F.H.  2015.  Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases. Biochemical Journal. 468:475-484.
  • Marous D.R, Lloyd E.P, Buller A.R, Moshos K.A, Grove T.L, Blaszczyk A.J, Booker S.J, Townsend C.A.  2015.  Consecutive radical S-adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 112:10354-10358.
  • Buller A.R, Brinkmann-Chen S., Romney D.K, Herger M., Murciano-Calles J., Arnold F.H.  2015.  Directed evolution of the tryptophan synthase beta-subunit for stand-alone function recapitulates allosteric activation. Proceedings of the National Academy of Sciences of the United States of America. 112:14599-14604.
  • McIntosh J.A, Heel T., Buller A.R, Chio L., Arnold F.H.  2015.  Structural Adaptability Facilitates Histidine Heme Ligation in a Cytochrome P450. Journal of the American Chemical Society. 137:13861-13865.


  • Hyster T.K, Farwell C.C, Buller A.R, McIntosh J.A, Arnold F.H.  2014.  Enzyme-Controlled Nitrogen-Atom Transfer Enables Regiodivergent C-H Amination. Journal of the American Chemical Society. 136:15505-15508.
  • Buller A.R, Freeman M.F, Schildbach J.F, Townsend C.A.  2014.  Exploring the Role of Conformational Heterogeneity in cis-Autoproteolytic Activation of ThnT. Biochemistry. 53:4273-4281.


  • Buller A.R, Townsend C.A.  2013.  Intrinsic evolutionary constraints on protease structure, enzyme acylation, and the identity of the catalytic triad. Proceedings of the National Academy of Sciences of the United States of America. 110:E653-E661.


  • Buller A.R, Labonte J.W, Freeman M.F, Wright N.T, Schildbach J.F, Townsend C.A.  2012.  Autoproteolytic Activation of ThnT Results in Structural Reorganization Necessary for Substrate Binding and Catalysis. Journal of Molecular Biology. 422:508-518.
  • Buller A.R, Freeman M.F, Wright N.T, Schildbach J.F, Townsend C.A.  2012.  Insights into cis-autoproteolysis reveal a reactive state formed through conformational rearrangement. Proceedings of the National Academy of Sciences of the United States of America. 109:2308-2313.


  • Bassuk A.G, Wallace R.H, Buhr A., Buller A.R, Afawi Z., Shimojo M., Miyata S., Chen S., Gonzalez-Alegre P., Griesbach H.L et al..  2008.  A Homozygous Mutation in Human PRICKLE1 Causes an Autosomal-Recessive Progressive Myoclonus Epilepsy-Ataxia Syndrome. American Journal of Human Genetics. 83:572-581.